Research

12 Sep 2019
  • Research Result

An overactive cerebellum causes issues across the brain

Acute cerebellar inflammation and hyperexcitability induces 'depression-like' behavior

Japan -- Consider the cerebellum. That structure tucked into the lower back of your skull. Also known as the 'little brain' it plays a key role in regulating voluntary movement like balance, motor learning, and speech.

Recent evidence even shows the cerebellum involved in higher-order brain functions including visual response, emotion, and motor planning. And now, a team from Kyoto University has found another link, depressive behavior.

Writing in Cell Reports, the research team found -- through a series of experiments with rats -- that acute cerebellar inflammation puts the structure in an 'overexcited' state, resulting in the animal developing a temporary decrease in motivation and sociability.

Team leader Gen Ohtsuki of Kyoto University's Hakubi Center for Advanced Research explains that the investigation began in an effort to understand how the brain's immune system can change its activity. In fact, literature has shown correlations between cerebellar dysfunction and certain pervasive developmental disorders such as autism and depression.

"Even though we now know more about the cerebellum's role in higher order brain functions the detailed signal transduction machinery remains a mystery. We know even less about what happens in the brain during excessive immune activity." explains Ohtsuki. "So, we conducted series of experiments where we activated the immune cells in the cerebellum and observed the results."

The brain's immune cells are known as microglia, and they respond to bacteria and viruses to mitigate damage. That response results in inflammation. Utilizing electrophysiological techniques, the team found that microglia caused neurons to fire at an increased rate, a phenomenon known as 'intrinsic-plasticity'. This in turn caused the cerebellum to go into a 'hyperexcited' state.

This immune-triggered response was shown to even change behavior. When rats were induced with acute cerebellar inflammation, their sociability, free-searching, and motivation dramatically decreased.

"These behavioral modulations are signs of 'depression-like' behavior. Once the inflammation subsided, they were back to normal," Ohtsuki continues. "Moreover, the phenotype can be rescued if the rats are treated with neuro-immunity suppressants and inflammatory cytokines. We also investigated if higher order brain regions were affected. fMRI studies on the rats show a clear increase in activity in the prefrontal cortex, highlighting the interconnectedness of the cerebellum to higher order brain regions."

The team is encouraged of their results, but states that further investigation is needed.

"Excessive immune activity in the brain can induce behavioral pathology, and we expect it to be involved in other mental and cognitive disorders such as dementia. But to understand anything about the pathological mechanisms we need to combine this with additional data such as genetic risk factors," concludes Ohtsuki. "In this study, we focused on inflammation. In the future, we will begin firmly clarifying the physiological, molecular, and genetic aspects of these behavioral changes."

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Rats with acute cerebellar inflammation have reduced sociability, free-searching, and motivation indicating 'depression-like' behavior (Kyoto University/Gen Ohtsuki)

Paper Information

【DOI】https://doi.org/10.1016/j.celrep.2019.07.078

Masamichi Yamamoto, Minsoo Kim, Hirohiko Imai, Yamato Itakura, Gen Ohtsuki (2019). Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation. Cell Reports, 28(11), 2923-2938.e8.