Fungus has a host of issues

KyotoU discovers how toxic fungi target specific host plants
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Kyoto, Japan -- While infectious fungal plant diseases relentlessly wreak havoc on many crops, they are also picky when choosing their hosts. Each fungus generally exhibits a specific host range, but the mechanism of this specificity has not been well understood.

Kyoto University researchers have now identified and categorized four fungal proteins called effectors responsible for suppressing host plant immunity from infection.

Phytopathogens are plant pathogenic fungi that secrete these effectors. As a result, the harmful fungus generally exhibits distinct host specificity when infecting plants, causing more than 70% of plant diseases.

According to the researchers, the four effector proteins named EPC1 to EPC4 in Colletotrichum orbiculare -- a phytopathogenic fungus -- establish specificity on a host cucumber.

"The four effectors have very different amino acid sequences, suggesting that they work independently, but we need to continue analyzing the function of each effector to get a clearer picture," says lead author Yoshitaka Takano of KyotoU's Graduate School of Agriculture.

Takano's team used functional analysis -- targeted gene disruption -- on effector-like genes, which are highly expressed in six isolates of the inoculated pathogen.

"We have just cracked open a peephole to understand how effectors of phytopathogenic fungi shape host specificity. Our growing knowledge may lead to new crop protection technologies."

Caption: Phytopathogenic fungus on cucumber leaf
Credit: KyotoU Tobiyama/Yoshitaka Takano
Caption: Deletion of four genes (EPCI to EPC4) reduced pathogen virulence. From left to right: cucumber host, melon host, and benth host
Credit: KyotoU Yoshitaka Takano
Takano, Yoshitaka
Publication information



Yoshihiro Inoue, Trinh Thi Phuong Vy, Suthitar Singkaravanit-Ogawa, Ru Zhang, Kohji Yamada, Taiki Ogawa, Junya Ishizuka, Yoshihiro Narusaka, Yoshitaka Takano (2023). Selective deployment of virulence effectors correlates with host specificity in a fungal plant pathogen. Selective deployment of virulence effectors correlates with host specificity in a fungal plant pathogen. New Phytologist, 238(4), 1578-1592.